Scanning electron microscope and Luminol/DNA as tools in the elucidation of a sniper case in São Paulo


Abstract

The maxim of the Locard Principle, that every contact leaves a mark, is very popular among forensic professionals, but it is often not exercised in its broadest sense. Usually, the analysis of a crime scene is restricted to the search for traces left by the perpetrator, ignoring possible elements present in the environment that were transferred to the criminal. This case report aims to demonstrate the practical use of this principle in which it was possible to establish the correlation of a suspect with a place of completed double homicide and attempted homicide with the use of a firearm. The object of analysis was the suspect's footwear, using the Luminol test for blood testing, DNA analysis of the chemiluminescent spots using Luminol and scanning electron microscopy for looking at glass fragments. Both pointed to elements from the crime scene present on the sole of the suspect's shoes, indicating that he was at the scene of the facts during/after the crime, stepping on the blood of one of the fatal victims and on the broken glass at the entrance to the establishment. Such results prove the need to pay attention to micro traces that may be present with suspects, especially in footwear.


Keywords

Locard Principle
Luminol
Scanning electron microscopy
Micro traces
Luminol;
Microscópio eletrônico de varredura
Micro vestígios
Princípio de Locard

References

  1. C.R. Dias et al. Introdução à Genética Forense. 3ª ed – Campinas: Editora Millennium, 2020.
  2. D. Tocchetto; L. E. Dorea; V. Quintela; V. P. Stumvoll. Tratado de Perícias Criminalísticas. 5ª ed. – Campinas: Editora Millennium, 2012.
  3. C. T. M. Damasceno; J. A. Velho; K. A. Costa. Locais de Crime: Dos vestígios à dinâmica criminosa. Campinas: Editora Millennium, 2013.
  4. F. Barni et al. Forensic application of the luminol reaction as a presumptive test for latent blood detection. Talanta 72(3), 896-913, 2007.
  5. M. Scimeca, et al. Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis. European Journal of Histochemistry 62(1), 2018.
  6. F.B.H. Dantas. Microscopia eletrônica de varredura aplicada à avaliação de embalagens de vidro. Informativo CETEA, Campinas, 16(2), 2004.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Copyright (c) 2023 Brazilian Journal of Criminalistics